BRAF and neoplasm: High-throughput sequencing of DNA isolated from the tumor and specifically enriched for putative oncogenic drivers and markers of solid tumors in children (the QIAseq customized panel protocol; Qiagen, Germany) revealed somatic double mutation in BRAF exon 15 (RefSeq NM_004333.6): c.1799T>A: p.V600E with 31% variant allele frequency (VAF) and c.1819T>A: p.S607T with 32% VAF in cis-position (Figure 2C, 2D).