PRKCI and osteosarcoma: Figures 2A–D show that PRKCI was overexpressed in osteosarcoma cells after plasmid transfection. Figures 2E, F show that osteosarcoma cells transfected with the PRKCI plasmid had stronger proliferation ability than cells transfected with an empty vector (p < 0.05), which was time-dependent. From Figures 2G–J, we learned that the number of clones in the PRKCI-overexpression group was significantly higher than that in the empty vector group (p < 0.01), indicating that the overexpression of PRKCI significantly increased the colony-forming capacity of cells (SW1353 and U2OS).