PRKCI and osteosarcoma: Figure 5 shows that, compared with control groups, overexpression of PRKCI could promote the migration and invasion of osteosarcoma cell lines SW1353 and U2OS, while silencing of PRKCI reversed this effect (p < 0.01). A wound-healing assay is another commonly used method to evaluate cell migration. The results in Figure 6 revealed that PRKCI overexpression and PRKCI silencing showed the same effect as before in both cell lines.