Therefore, we detected CD4+ T cells in each group of tumor tissues using flow cytometry and the results are shown in Fig. 10c, the number of CD4+ T cells in the tumor tissues of tumor-bearing mice accounted for only 0.36% of the total number of cells in the saline group, while the number of CD4+ T cells in the other groups increased significantly, among them, CD4+ T cells accounted for the largest proportion in the PPH@CR group (2.55%), followed by the Cur + Rg3 group (1.1%), the PPH@C group (0.74%) and the Cur group (0.64%). This evidence concerns the gene CD4 and neoplasm.