HMGB1 and neoplasm: Studies have shown that both Cur and Rg3 can induce ICD in tumor cells but the effect of single agent inducing ICD in cells is weak [24], while nanodrug delivery systems can deliver multiple ICD inducers simultaneously to increase ICD effect [25] In this experiment, we evaluated the PPH@CR-induced ICD in tumor cells by measuring CRT exposure and ATP and HMGB1 release after drug action on 4T1 cells.