As shown in Fig. 9c, the fluorescence of HMGB1 in tumor tissues after saline treatment basically overlapped with the nucleus, which was due to the fact that HMGB1 is mainly expressed in the nucleus in normal cells, while HMGB1 in 4T1 cells will be transferred from the nucleus to the cytoplasm through the nuclear membrane after being treated with the drug and then released into the extracellular space through the plasma membrane [34]. The gene discussed is HMGB1; the disease is neoplasm.