After co-culture PHA-stimulated PBMCs and transfected NSCLC cells, IFN-γ and TNF‐α levels were elevated in PHA-stimulated PBMCs, and interference of circPTP4A2 further enhanced IFN‐γ and TNF‐α levels (Fig. 4A and B). This evidence concerns the gene TNF and non-small cell lung carcinoma.