To verify whether ALYREF is a functional m5C reader in lung cancer cells, we performed RIP assay with ALYREF antibody in H1650 and H1299 cells, and the RT-qPCR results showed that ALYREF could bind to EZH2 mRNA, suggesting that ALYREF might be the m5C reader (Fig. 5I). This evidence concerns the gene EZH2 and lung carcinoma.