To further investigate the effect of NACT ± P on T cells, we stained paired tumor samples from the 64 patients with a 7-color immunofluorescence panel (CD4, CD8, PD-1, Ki67, ActCasp3, panCK) to analyze changes in tumor cells and T cell subsets upon treatment (Supplementary Fig. 3A). This evidence concerns the gene CD4 and neoplasm.