These cells release cytokines like transforming growth factor-beta (TGFβ) and interleukin-10 (IL-10) within solid tumors, which diminish the effectiveness of CAR-T cells in fighting tumors.165 To overcome this, strategies include combining CAR-T cells with immune checkpoint inhibitors such as PD-1 inhibitors in immunotherapy and genetically modifying CAR-T cells to enhance their immune response and resistance to inhibitory factors, thereby improving their anti-tumor activity.166 Several clinical trials on the combination of CAR-T and immune checkpoints blockades are summarized in Table 3. This evidence concerns the gene IL10 and neoplasm.