To determine whether these IDRs affect DNA binding, we performed eY1H screens using wild-type ESR1, three truncations of the N-terminal IDR (ΔN59, ΔN119, and ΔN179), two truncations of the C-terminal IDR (ΔC23 and ΔC43), a replacement of the hinge region with 23 tandem copies of Gly-Ser (Hinge[GS]23) to maintain flexibility of the linker region while removing the endogenous sequence, and 11 cancer-associated mutations in these IDRs reported in COSMIC (Fig 5A, Table S4). This evidence concerns the gene ESR1 and cancer.