For induction of orthotopic GBM xenografts in nude mice, 7 × 105 (WT-GBM, VEGF-KO-GBM) or 3 × 105 (NCH421k, U87-MG) cells were implanted intracranially in the right striatum of the brain (coordinates: 2 mm laterally and 2 mm posteriorly from bregma and at 2.5 mm depth). The gene discussed is VEGFA; the disease is glioblastoma.