The results showed that 7 metabolic pathways were changed between AB43 and AB43ΔCRISPR-Cas under 0, 1/4 MIC CRO, 1/4 MIC LFX, and 1/4 MIC TET (Figure 3), including Linoleic acid metabolism, Biosynthesis of plant secondary metabolites, Central carbon metabolism in cancer, ABC transporters, Glycine, serine and threonine metabolism, Biosynthesis of amino acids, and beta-Alanine metabolism. This evidence concerns the gene ABCG2 and cancer.