In addition, it is important to note that prophylactic vaccination of mice with CX3CL1 alone (i.e., without any MTX-treated cancer cells) had no effect on the tumour-free survival of the mice (Supplementary Figure S1), logically due to the absence of any antigens (antigenicity) or other DAMPs (adjuvanticity) during vaccination. This evidence concerns the gene CX3CL1 and neoplasm.