In addition, it is important to note that prophylactic vaccination of mice with CX3CL1 alone (i.e., without any MTX-treated cancer cells) had no effect on the tumour-free survival of the mice (Supplementary Figure S1), logically due to the absence of any antigens (antigenicity) or other DAMPs (adjuvanticity) during vaccination. Here, CX3CL1 is linked to cancer.