ACTA1 and renal fibrosis: Next, we stepped forward to know the differences in the impacts of intrarenal MSU crystals, soluble UA, and UA+MSU on the degree of renal fibrosis through evaluation of the fibrosis marker proteins α-SMA and vimentin proteins of connective tissue fibers in renal tissue sections using Masson’s trichrome staining and immunohistochemical staining.