The upregulation of FTO obviously promotes cisplatin chemoresistance of gastric cancer cells (SGC-7901/DDP) whereas Knockdown of FTO significantly reverses cisplatin chemoresistance of SGC-7901/DDP cells both in vitro and in vivo, which was associated with the inhibition of Unc-51-like kinase 1 (ULK1)- mediated autophagy and the decrease of the m6 A methylated RNA level. Here, ULK1 is linked to gastric cancer.