Moreover, the upregulation of myogenesis hallmarks, such as the embryonic muscle gene MYH3, PCEN2, COL4A2, and DOK7 in RMS tumors, coupled with the downregulation of CHRNA4 (Niemeyer et al, 2001; Faravelli et al, 2014), N-Cadherin (CDH2) (Marthiens et al, 2002), LRRC4 (Deng and Wu 2023), and STXBP5 (Carr and Munson 2007) genes in both tumor types, which are implicated in synapse organization (GO:0045202) and neuromuscular junctions, was evidenced. This evidence concerns the gene CHRNA4 and neoplasm.