PTPA and infection: Treatment of iKD-PP2A-C2 or iKD-PP2A-A2 strains with rapamycin or anhydrotetracycline (ATc) for 48 h result in efficient protein downregulation (Fig. 9C,D), severely impacting on the lytic cycle of the parasites as no plaque could be observed 7 days post-infection (Fig. 9E,F).