Primary tumor organoids without CDH3 could sendout protrusions, but these protrusions did not adhere to the matrixto make a long-lasting protrusion and thus lost their ability to migratecollectively.57 These findings correlatewith our current study: in CDH3−/− cysts,we also did not observe any changes in cyst cell morphology parameters(roundness, aspect ratio, or orientation angle), with associated impairedcell spreading potential (shape factor). The gene discussed is CDH3; the disease is neoplasm.