Primary tumor organoids without CDH3 could sendout protrusions, but these protrusions did not adhere to the matrixto make a long-lasting protrusion and thus lost their ability to migratecollectively.57 These findings correlatewith our current study: in CDH3−/− cysts,we also did not observe any changes in cyst cell morphology parameters(roundness, aspect ratio, or orientation angle), with associated impairedcell spreading potential (shape factor). This evidence concerns the gene CDH3 and cyst.