To understand how MDCK cells respond to the aligned matrix, weperformed knockout studies of CDH3, a known cell–cell adhesionmarker that regulates collective invasion of breast tumor organoids.57 In our prior work, we demonstrated that CDH3has a regulatory role in primary breast tumor organoids, which areepithelial-derived.57 When we knocked downCDH3 in primary breast tumor organoids, we observed a loss of collectivemigration potential. The gene discussed is CDH3; the disease is breast neoplasm.