In all of these experiments, aged fibroblasts were able to increase invasion of melanoma cells but less so in the absence of IGFBP2, whereas rIGFBP2 treatment or IGFBP2 overexpression in melanoma cells was able to increase invasion of melanoma cells in both young CM or in the presence of young fibroblasts. The gene discussed is IGFBP2; the disease is melanoma.