Immunoblot analyses for the expression of NDUFB6, a gene found enriched in several of the datasets evaluated (Supplementary Fig. S2A–S2C), and ACAD9, a critical influencer on oxidative phosphorylation (37) also found enriched in the GSEA, were also found increased upon 3PO treatment in two of the three PDOs and in three of the four tested colorectal cancer cells (Figs. 3D and E; Supplementary Fig. S3A). The gene discussed is NDUFB6; the disease is colorectal cancer.