Stimulation of PBMC with 1B3-transfected tumor cells alone did not result in activation of CD4+ or CD8+ T cells, but CD4-CD8- T cells did show an increase in percentage IFNγ+ and TNFα+ cells suggesting that this population can respond to tumor cells directly (Figure 6G, Supplementary Figure 9E–9G). Here, CD4 is linked to neoplasm.