In order to confirm that OX40L/PPT complexes are capable of efficient cancer cell transfection, we have tested the in vitro transfection efficiency of the complexes using three murine cancer cell lines of different origin, CT26gfp (colon cancer), B16F0 (melanoma) and 4T1 (breast cancer). The gene discussed is TNFSF4; the disease is colonic neoplasm.