Additionally, the individual ROC curves for the six variables in the nomogram revealed that the area under the curve (AUC) for Log CYFRA21–1 (AUC = 0.850, 95% CI: 0.796–0.903) was greater than those for the other five variables, including Log CA19–9 (AUC = 0.780, 95% CI: 0.711–0.849) and Log AFP (AUC = 0.768, 95% CI: 0.720–0.817) (Figure 2B), demonstrating that CYFRA21–1 plays a significant role in the differential diagnosis of ICC and HCC. This evidence concerns the gene AFP and intrahepatic cholangiocarcinoma.