The role of SLC30A5 in HCC was evaluated through <i>in vitro</i> and <i>in vivo</i> assays, including CCK8 viability assay, EdU cell proliferation assay, colony formation assay, apoptosis assay, wound healing assay, transwell migration assay, and xenograft mouse model assay using Huh7 cells with targeted knockdown of SLC30A5. This evidence concerns the gene SLC30A5 and hepatocellular carcinoma.