Regarding cytokine expression, qRT-PCR analysis revealed that mRNA expression levels of TNF-α and IL-6 in the liver were significantly decreased in treated PCOS rats at the doses of 200 and 400 mg/kg P. anisum compared to the PCOS group (Figure 5C and D, P < 0.001). This evidence concerns the gene IL6 and polycystic ovary syndrome.