GLP1R and type 2 diabetes mellitus: Building on our ESI analysis, which indicated that GLP1R is one of the T2D-associated genes enriched in our hypothalamic neurons on day 28 (Fig. 4F), we first measured GLP1R transcript expression using RT-qPCR under different culture conditions: low glucose (1 g/L), medium glucose (4 g/L), or high glucose (9 g/L) supplemented Arc-3 medium for 96 h (Fig. 7E).