Furthermore, overexpression of YAP1‐5SA, an activated mutant of YAP1 with deficient phosphorylation, could reverse the cytoplasmic localization caused by silencing of TRIM65 (Figure 4G,H; Figure S4D,F, Supporting Information), indicating that TRIM65 participates in the upstream activation of YAP1 in HCC cells. The gene discussed is YAP1; the disease is hepatocellular carcinoma.