In particular, we confirmed that nuclear extracts and the nuclear localization signal of YAP1 from the liver tissues of Trim65 cKO mice were decreased in DEN/CCl4 and MET/N90 HCC models (Figure 4I,J; Figure S4H,I, Supporting Information), which was consistent with the delayed HCC progression. Here, TRIM65 is linked to hepatocellular carcinoma.