Subsequently, we constructed an in situ HCC model[29] using Sleeping Beauty (SB) transposase along with pT3‐cMet and pT3‐ΔN90‐β‐catenin or equivalent saline by hydrodynamic tail vein injection (HTVi) in Trim65fl/fl and Trim65fl/fl;Alb‐Cre mice respectively (MET/N90 for short) (Figure 2A; Figure S3I, Supporting Information) and survival analysis revealed that Trim65 expression led to a poor prognosis implying a more severe tumor burden (Figure 2B). The gene discussed is TRIM65; the disease is hepatocellular carcinoma.