After separating the cytoplasm and nuclei of DLBCL-resistant cells, it was found that the knockdown of PDK4 could significantly downregulate the expression levels of nuclear PDK4 and phosphorylated HDAC8 but upregulate the cytoplasmic expression of CD20 (Fig. 2C), where Histone H3 was used as an internal reference for the nucleus. This evidence concerns the gene HDAC8 and diffuse large B-cell lymphoma.