In our study, MMP13 and ADAMTS5 expressions in ALN + Loaded group were significantly lower than Saline + Loaded and unloaded control groups (Figs. 7 and 8), indicating that ALN may downregulate key cartilage degeneration markers, which possibly explained the restoration of cartilage thickness and proteoglycan distance by ALN (Figs. 5 and 6).Overall, these observations lead us to conclude that TMJ cartilage was very responsive to ALN treatment in OI TMJ loading model. Here, ADAMTS5 is linked to osteogenesis imperfecta.