HOXA9 and acute myeloid leukemia: To identify the corresponding subpopulation containing LICs in Hoxa9-only-driven AML, and to characterize the differentiation hierarchy of this model, we settled on a strategy using c-Kit and Ly6C expression to divide GFP+ marrow cells into three populations: (i) cKit+ Ly6C-, (ii) c-Kit+ Ly6C+, and (iii) c-Kit- Ly6C+ (Fig. 3A, S5B).