Given that B3galt5 was highly expressed in colon, to further confirm the intestinal B3galt5 function influencing metabolic disorders, we generated intestinal epithelial cell-specific B3galt5 knockout mice (B3galt5△IEC) by intercrossing B3galt5f/f mice with Villin-Cre-ERT mice (Figure S8a). This evidence concerns the gene B3GALT5 and metabolic disease.