The expression levels of these pivotal genes were validated using the POF-related dataset GSE48873 and DED-related dataset GSE171043, suggesting that CDC20, CDCA8, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, and CDCA3 are potential comorbid genes in POF and DED. This evidence concerns the gene TIPIN and premature menopause.