The expression levels of these pivotal genes were validated using the POF-related dataset GSE48873 and DED-related dataset GSE171043, suggesting that CDC20, CDCA8, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, and CDCA3 are potential comorbid genes in POF and DED. Here, CDCA8 is linked to premature menopause.