To examine whether this interaction between NHE6 and GGA1 could be detected endogenously without overexpression of constructs, SH-SY5Y neuroblastoma cell lysates were precipitated with either anti-NHE6 antibody or control-immunoglobulin G (IgG), followed by immunoblotting with anti-GGA1 antibody. This evidence concerns the gene GGA1 and neuroblastoma.