We sought to evaluate the effect of decreased PFKFB3 expression in a tumor model that accurately recapitulates human disease and therefore employed a model of spontaneous oncogene-driven mammary tumor development that relies on the transgenic expression of activated Erbb2 (HER2/neu) under the control of the mouse mammary tumor virus (Erbb2 mice) [35]. This evidence concerns the gene PFKFB3 and breast cancer.