EGFR and brain cancer: Conversely, there was a significant negative correlation between EGFR alterations and GCLC mRNA expression, (n = 244, Pearson correlation= −0.19, p = 3.00 × 10−3), as shown in Figure 3H. Thus, in accordance with relatively low intracellular GSH in U87/EGFRwt and U87/EGFRvIII compared to the U87MG cell line, EGFR and GCLC co-expression showed a significant decrease in EGFR-overexpressing brain cancer tissue compared to normal brain tissue.