Liver fibrosis in the high-fat high-cholesterol (HFHCD) model is mediated through an increase in gene expression for TGF-β1, αSMA, and collagen type I. During fibrogenesis, other genes involved in ECM remodeling are highly expressed in liver tissue, such as MMP2 and MMP9. The gene discussed is ACTA1; the disease is Hepatic fibrosis.