A study using large mammal beagles as a DKD model confirmed an association between ERS and thermoprotein deposition during the pyroptosis process of DKD following the addition of 4-phenylbutyric acid (4-PBA) and BYA 11-7082 to high-glucose (HG)-treated MDCK (Martin d’Arby’s dog kidney) cells, and that ERS inhibitor 4-Phenylbutytic acid (4-PBA) and NF-κB inhibitor BYA11–7082 decreased the expression of NLRP3 and GSDMD in renal cells, suggesting that ERS alleviated the high-glucose-induced pyroptosis in Madin–Daby canine kidney (MDCK) cells through the NF-κB/NLRP3 pathway [70]. This evidence concerns the gene NFKB1 and diabetic kidney disease.