AKT1 and breast cancer: Western blot analysis was performed for p-AKT (Ser473), p-AKT1S1 (Thr246), and p-mTOR (Ser2448) in the HR+ breast cancer cell lines MCF-7, ZR-75, and MCF-7-Y537S following exposure to 10 dyn/cm2 of FSS using the microfluidic device followed by seeding in culture and growth on TCP.