To examine the effect of inflammatory cytokines, we treated the cells with HMGB1 and TNFα for 48 h at concentrations equivalent to those in the cancer ascites (50 μg/mL and 35 pg/mL, respectively) and examined the changes in 4HNE, PINK1, and Parkin (Figure 3B). The gene discussed is HMGB1; the disease is cancer.