Finally, we analyzed the ability of GAS6 and SPP1 as glaucoma aqueous humor biomarkers to discriminate glaucoma patients from ICL and cataracts controls by ELISA using a collection of 162 individual aqueous humors samples (39 ICL, 92 cataracts, and 31 glaucoma samples) (Figure 1). This evidence concerns the gene SPP1 and glaucoma.