DUX4 and facioscapulohumeral muscular dystrophy: Indeed, we have proven that berberine treatment in FSHD patient-derived muscle cells and in mice, injected intramuscularly with adeno-associated viral vectors expressing exogenous DUX4, decreased expression of DUX4 and its downstream targets, and improved muscle histopathology and muscle strength of tibialis anterior (TA) muscle, compared to untreated controls [20].