In this study, we investigated frequencies of leukemia-specific cells in uncultured AML-patients’ immune cells (with and without additional stimulation with LAA) and, moreover, the effects of Kit M pretreated whole blood (WB) after MLC on the generation of leukemia-specific cells, with a focus on DEG and INCYT (INFy and TNFa) assays, followed by a correlation of results with cytotoxicity assays and diagnostic/clinical data. This evidence concerns the gene TNF and acute myeloid leukemia.