To validate the diagnostic value of the key genes in AD, we established ROC curves through dataset GSE132903, revealing high diagnostic values for LEP (AUC: 0.721229), PLXNA3 (AUC: 0.742689), HLA-E (AUC: 0.691248), DDX58 (AUC: 0.754103), IKBKE (AUC: 0.733537), VCAM1 (AUC: 0.635283), HSPA6 (AUC: 0.678098), SOS2 (AUC: 0.686409), and HSPA1A (AUC: 0.615927) in AD (Figure S1A). This evidence concerns the gene PLXNA3 and Alzheimer disease.