In order to reveal the role of NM proteins in the transition to CRPC, we performed focused a 2D-proteomic analysis of the NM proteins using an androgen-sensitive prostate cancer cell line, LNCaP, and its derivative, LN96, which were established after long-term culturing under androgen-deprived conditions, as a representative model of the transition states, and we reveal that purine-rich element binding protein alpha (PURα) was detected in the NM proteins and differentially expressed between the cell lines [13]. Here, GNE is linked to Familial prostate cancer.