On the other hand, studies in vitro in prostate cancer cells have shown the upregulation of several ZIPs, including ZIP4, ZIP6, and ZIP8, in different prostate cancer cell lines that represent different metastatic origins (LNCaP from the lymph node, PC3 from the bone, and DU145 from the brain), and tumorigenic potential (LNCaP, very low; PC3, moderated; and DU145, high) [40,70,76], but their pathological mechanisms are largely unknown in prostate cancer. This evidence concerns the gene SLC39A8 and Familial prostate cancer.