Consequently, for individuals without p.C282Y mutation and no signs and symptoms of iron overload, the genes sequencing tests of the basic panel are recommended and should cover the following genes: rare HFE gene mutation, caeruloplasmin (CP gene), bone morphogenetic protein (BMP6 gene), solute carrier family 40 member 1 (SLC40A1 gene), the genes related to the early disease onset as HAMP gene and hemojuvelin (HJV gene) and those connected with abnormal transferrin, transferrin receptor 2 (TFR2 gene) and transferrin (TF gene), and the hyperferritin-cataract syndrome gene (FTL gene). This evidence concerns the gene TF and Down syndrome.