In addition, further studies have shown that DPPH can increase acetyltransferase (CHAT) activity in the cerebral cortex and hippocampus, and reduce acetylcholinesterase (AChE) activity and increase acetylcholine content in brain tissue, thereby improving Aβ1–40-induced learning and memory impairment in mice [64] (the possible detailed mechanism of action is shown in Figure 8). This evidence concerns the gene ACHE and memory impairment.