A rougher surface was detected for TF (0.82 ± 0.21 μm) compared to BRF (0.58 ± 0.04 μm) (p < 0.05), whilst CRS (0.78 ± 0.15 μm) demonstrated no significant difference from TF or BR (p > 0.05). This evidence concerns the gene TF and congenital rubella syndrome.