Conversely, in order to ascertain if the reduction in CXCL2 observed in HCC [180, 181] was regulated by DNA methylation, the CXCL2 levels of HCC cell lines were elevated after treatment with the DNA demethylating agent 5-aza-2′-deoxycytidine [181]. This evidence concerns the gene CXCL2 and hepatocellular carcinoma.