The HCC vaccine developed based on Listeria can upregulate NF-κB via TLR-2 and MyD88 overexpression, and it mediates p62 recruitment to induce the autophagy axis, causing M1 polarization of macrophages in sensitizing HCC cells to anti-PD-1 therapy [119]. This evidence concerns the gene TLR2 and hepatocellular carcinoma.