It has been reported that bufalin is able to reduce upregulation of p50 to increase accumulation of p65–p50 heterodimers in nucleus and mediates a predominance over p50 homodimer in nucleus, resulting in NF-κB induction that mediates M1 polarization of macrophages and increases infiltration of T cells in TME for enhancing anti-tumor immunity [117]. This evidence concerns the gene NFKB1 and neoplasm.