Consistently, analysis of a clinical scRNA-seq dataset that contains 31 breast cancer patients30 also demonstrated that patients with low expression of TSs from the TS-neg-Immune list had more PD1+ LAG3+ CD4 T cells and CTLA4+ Treg as well as PD1+ LAG3+ CD8 T cells (Fig. 1D and Fig. S1D). Here, CTLA4 is linked to breast cancer.