Increased tumor infiltration by monocytes, classified by high Ly6c1 expression, was observed across all treatment groups as compared to sham control mice (Fig. 5f), with an enrichment of non-classical monocytes characterized by Spn, Cx3cr1, and Tnfrsf1b expression37 in groups receiving anti-PD-1 treatment (Fig. 5g). Here, CX3CR1 is linked to neoplasm.