CD4 and amyotrophic lateral sclerosis: Since our intervention transiently disrupted the BSCB, glial cell immunoreactivity was increased, microglial RNAseq analyses highlighted beside decreased proliferative pathways also increased pathways regulating T cells, and as CD4+ T cells are known to be protective in this ALS mouse model,42 we further examined the extent of lymphocyte infiltration in the different groups (Fig. 6a-n).